Hotspot

trnT-GGU-trnE-UUC

LSC · 20446-21136 · 691 bp

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Pi 0.0134
MarkerSeek score 60.60
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
691
variable_sites
18
pi
0.0134
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 GAACCGATGACTTACGCCTT TTTCGTAGTACCCTACCCCC 50.0 55.0 58.0 57.8 828-846 bp 1.0 81.0
trnT-GGU-trnE-UUC_p2 CAGACCGGAGTTTTGTGGAA TTTCGTAGTACCCTACCCCC 50.0 55.0 58.0 57.8 867-885 bp 1.0 80.6
trnT-GGU-trnE-UUC_p3 TTCAGACCGGAGTTTTGTGG TTTCGTAGTACCCTACCCCC 50.0 55.0 58.0 57.8 869-887 bp 1.0 80.6
trnT-GGU-trnE-UUC_p4 TACCGCTGAGTTAAAAGGGC TTTCGTAGTACCCTACCCCC 50.0 55.0 57.9 57.8 793-811 bp 1.0 80.3
trnT-GGU-trnE-UUC_p5 GAACCGATGACTTACGCCTT GCTGCCTCCTTGAAAGAGAG 50.0 55.0 58.0 58.3 790-808 bp 1.0 80.3