Hotspot

trnS-GGA-ycf3

LSC · 6398-7269 · 872 bp

Back to Adonis

Pi 0.0161
MarkerSeek score 70.80
Primer available yes
Label trnS-GGA-ycf3

Feature Metadata

label_name
trnS-GGA-ycf3
feature_type
igs
region
LSC
length_bp
872
variable_sites
29
pi
0.0161
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-ycf3_p1 TGTTCGTGACTTAGGCGAAG CATTTATCCGCGATCTAGGCA 50.0 47.6 57.9 58.0 988-1023 bp 1.0 75.6
trnS-GGA-ycf3_p2 CCCTCGGTAAACAAAAGCCT CATTTATCCGCGATCTAGGCA 50.0 47.6 58.1 58.0 945-980 bp 1.0 75.5
trnS-GGA-ycf3_p3 GCGAAGGTACGGAAAGAGAG CATTTATCCGCGATCTAGGCA 55.0 47.6 58.1 58.0 974-1009 bp 1.0 75.4
trnS-GGA-ycf3_p4 CGTGACTTAGGCGAAGGTAC CATTTATCCGCGATCTAGGCA 55.0 47.6 58.1 58.0 984-1019 bp 1.0 75.3
trnS-GGA-ycf3_p5 GTGACTTAGGCGAAGGTACG CATTTATCCGCGATCTAGGCA 55.0 47.6 58.1 58.0 983-1018 bp 1.0 75.3