Hotspot

psbB

LSC · 73914-75440 · 1527 bp

Back to Adinandra

Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CCACTGAACAAGGGAGATCC	AGGCGAAAGATACCAAAGCA	55.0	45.0	57.6	57.5	1647-1655 bp	1.0	72.9
psbB_p2	CCACTGAACAAGGGAGATCC	GAGGCGAAAGATACCAAAGC	55.0	50.0	57.6	56.9	1648-1656 bp	1.0	67.9
psbB_p3	CCACTGAACAAGGGAGATCC	AGGCGAAAGATACCAAAGCAA	55.0	42.9	57.6	58.2	1647-1655 bp	1.0	67.2
psbB_p4	CACTGAACAAGGGAGATCCA	AGGCGAAAGATACCAAAGCA	50.0	45.0	56.8	57.5	1646-1654 bp	1.0	67.0
psbB_p5	CCACTGAACAAGGGAGATCC	GGCGAAAGATACCAAAGCAA	55.0	45.0	57.6	56.7	1646-1654 bp	1.0	66.5