Hotspot

ndhH-trnN-GUU

SSC · 124909-131573 · 6665 bp

Back to Adinandra

Pi 0.0026
MarkerSeek score 46.20
Primer available yes
Label ndhH-trnN-GUU

Feature Metadata

label_name
ndhH-trnN-GUU
feature_type
igs
region
SSC
length_bp
6665
variable_sites
26
pi
0.0026
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-trnN-GUU_p1 TGTTGATTCAAGAAAAAGTTGT TCACTGATCGAACAAGCACA 27.3 45.0 53.0 57.5 283-284 bp 1.0 40.8
ndhH-trnN-GUU_p2 TTGTTGATTCAAGAAAAAGTTGT TCACTGATCGAACAAGCACA 26.1 45.0 53.8 57.5 284-285 bp 1.0 40.8
ndhH-trnN-GUU_p3 TTTGTTGATTCAAGAAAAAGTTGT TCACTGATCGAACAAGCACA 25.0 45.0 54.6 57.5 285-286 bp 1.0 40.8
ndhH-trnN-GUU_p4 TGTTGATTCAAGAAAAAGTTGT TCTCACTGATCGAACAAGCA 27.3 45.0 53.0 56.9 285-286 bp 1.0 40.8
ndhH-trnN-GUU_p5 TGTTGATTCAAGAAAAAGTTGT CTCACTGATCGAACAAGCAC 27.3 50.0 53.0 56.9 284-285 bp 1.0 40.8