Hotspot

trnR-ACG-trnN-GUU

IRb · 109750-110332 · 583 bp

Back to Adinandra

Pi 0.00686

MarkerSeek score 62.80

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 583
variable_sites: 6
pi: 0.00686
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	724 bp	1.0	78.9
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	761 bp	1.0	78.3
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	765 bp	1.0	78.1
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	675 bp	1.0	77.7
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	802 bp	1.0	77.5