Hotspot

ndhC-trnM-CAU

LSC · 52018-53167 · 1150 bp

Back to Adinandra

Pi 0.00232

MarkerSeek score 60.30

Primer available yes

Label ndhC-trnM-CAU

Feature Metadata

label_name: ndhC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 4
pi: 0.00232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAU_p1	GGGCTAAAACCCCGGAAATA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.6	58.4	1290-1291 bp	1.0	73.5
ndhC-trnM-CAU_p2	TCCTTTCCTAATCGGGGCTA	CCCGCCGTATGAAAGCAATA	50.0	50.0	57.5	58.4	1304-1305 bp	1.0	73.2
ndhC-trnM-CAU_p3	CTGGTCCTTTCCTAATCGGG	CCCGCCGTATGAAAGCAATA	55.0	50.0	57.4	58.4	1308-1309 bp	1.0	72.0
ndhC-trnM-CAU_p4	GGGCTAAAACCCCGGAAATA	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.6	57.3	1289-1290 bp	1.0	71.5
ndhC-trnM-CAU_p5	TCCTTTCCTAATCGGGGCTA	CCGCCGTATGAAAGCAATAC	50.0	50.0	57.5	57.3	1303-1304 bp	1.0	71.1