Hotspot

psaA-ycf3

LSC · 42622-43354 · 733 bp

Back to Adinandra

Pi 0.00728

MarkerSeek score 50.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 8
pi: 0.00728
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CTTCTGGTTCCGGCGAAC	GGAAACAGGCTATAGCGCTT	61.1	50.0	58.4	58.0	841-844 bp	1.0	60.9
psaA-ycf3_p2	CTTCTGGTTCCGGCGAAC	AGGCTATAGCGCTTACTCCT	61.1	50.0	58.4	57.7	835-838 bp	1.0	58.9
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	TTGGAAACAGGCTATAGCGC	61.1	50.0	58.4	58.3	843-846 bp	1.0	58.6
psaA-ycf3_p4	CTTCTGGTTCCGGCGAAC	GCTATAGCGCTTACTCCTGG	61.1	55.0	58.4	57.6	833-836 bp	1.0	58.4
psaA-ycf3_p5	CTTCTGGTTCCGGCGAAC	GGCTATAGCGCTTACTCCTG	61.1	55.0	58.4	57.6	834-837 bp	1.0	58.4