Hotspot

trnT-GGU-psbD

LSC · 32621-33677 · 1057 bp

Back to Adinandra

Pi 0.00379

MarkerSeek score 61.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 6
pi: 0.00379
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1217-1224 bp	1.0	79.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1220-1227 bp	1.0	79.0
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	AAAGAGCAGTAAACCGGACC	55.0	50.0	58.1	57.8	1232-1239 bp	1.0	78.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	AAAGAGCAGTAAACCGGACC	50.0	50.0	58.1	57.8	1235-1242 bp	1.0	78.4
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1207-1214 bp	1.0	78.1