Hotspot

trnK-UUU-rps16

LSC · 4322-5187 · 866 bp

Back to Adinandra

Pi 0.00385
MarkerSeek score 63.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
866
variable_sites
5
pi
0.00385
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 ATGGACGAATCCCTTGCTTC TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.0 58.0 1040-1056 bp 1.0 81.2
trnK-UUU-rps16_p2 GGACGAATCCCTTGCTTCAT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.0 58.0 1038-1054 bp 1.0 81.2
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC AGAAGGCGGGGGTTTTTAAG 50.0 50.0 58.0 58.1 990-1006 bp 1.0 80.7
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT AGAAGGCGGGGGTTTTTAAG 50.0 50.0 58.0 58.1 988-1004 bp 1.0 80.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.2 58.0 986-1002 bp 1.0 80.3