Hotspot

rps16

LSC · 3621-4283 · 663 bp

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Pi 0.238

MarkerSeek score 82.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 663
variable_sites: 298
pi: 0.238
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCTTCCACTTTTGCCTTCCC	ACGTATTGACATCCCGTGTG	50.0	50.0	58.0	58.0	1496 bp	0.2	47.9
rps16_p2	TCTTCCACTTTTGCCTTCCC	CACGACACCAAGGGGATAAG	50.0	55.0	58.0	58.0	1327 bp	0.2	47.8
rps16_p3	TCTTCCACTTTTGCCTTCCC	CTGAACGGGTGGCATCATAA	50.0	50.0	58.0	58.0	1032 bp	0.2	47.7
rps16_p4	TCTTCCACTTTTGCCTTCCC	CAGGTTCAGGGTTTGTGTGA	50.0	50.0	58.0	57.9	1618 bp	0.2	47.6
rps16_p5	TCTTCCACTTTTGCCTTCCC	GGGTTACGAGGGAACGAATC	50.0	55.0	58.0	58.1	1418 bp	0.2	47.4