Hotspot

rpl23

LSC · 81626-81907 · 282 bp

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Pi 0.19

MarkerSeek score 72.50

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: LSC
length_bp: 282
variable_sites: 102
pi: 0.19
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	GCGTTTGGGCTGGATAAAAC	TTGTTTTCATCTCCCGCCAA	50.0	45.0	58.0	58.0	1282 bp	0.2	47.8
rpl23_p2	GCGTTTGGGCTGGATAAAAC	TTTGTTTTCATCTCCCGCCA	50.0	45.0	58.0	58.0	1283 bp	0.2	47.8
rpl23_p3	TCCCCCAACTTCGTACTTCT	TTGTTTTCATCTCCCGCCAA	50.0	45.0	58.0	58.0	886 bp	0.2	47.7
rpl23_p4	TCCCCCAACTTCGTACTTCT	TTTGTTTTCATCTCCCGCCA	50.0	45.0	58.0	58.0	887 bp	0.2	47.7
rpl23_p5	GCGTTTGGGCTGGATAAAAC	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.0	58.0	1021 bp	0.2	47.7