Hotspot

rpl16

LSC · 77181-78274 · 1094 bp

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Pi 0.146

MarkerSeek score 83.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1094
variable_sites: 307
pi: 0.146
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CCCCCTACGAGTGCATACTA	AAGACCTGGGTATTTCGGGA	55.0	50.0	58.0	58.0	2002 bp	0.2	47.6
rpl16_p2	AAAACATGTTGGGTACGGCT	AAGACCTGGGTATTTCGGGA	45.0	50.0	58.0	58.0	1952 bp	0.2	47.6
rpl16_p3	AAACATGTTGGGTACGGCTT	AAGACCTGGGTATTTCGGGA	45.0	50.0	58.0	58.0	1951 bp	0.2	47.6
rpl16_p4	ATTTCCCCTTCCCCGTAAGA	AAGACCTGGGTATTTCGGGA	50.0	50.0	58.0	58.0	1540 bp	0.2	47.5
rpl16_p5	CCCCCTACGAGTGCATACTA	GTAGTGAAATGGCTCGCGTA	55.0	50.0	58.0	58.1	2121 bp	0.2	47.3