Hotspot

ndhF

SSC · 105718-107907 · 2190 bp

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Pi 0.183

MarkerSeek score 79.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2190
variable_sites: 768
pi: 0.183
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAGGTCAAGATCAACCTGG	CCCGGTTGTACTCGTAGTTG	55.0	55.0	58.0	58.0	2799 bp	0.2	47.8
ndhF_p2	CGAGGTCAAGATCAACCTGG	TCGTTCACAAAGCGAGTTGA	55.0	45.0	58.0	58.1	2688 bp	0.2	47.3
ndhF_p3	GGTCAAGATCAACCTGGCAA	CCCGGTTGTACTCGTAGTTG	50.0	55.0	58.1	58.0	2796 bp	0.2	47.2
ndhF_p4	CGAGGTCAAGATCAACCTGG	TCCCGGTTGTACTCGTAGTT	55.0	50.0	58.0	58.1	2800 bp	0.2	47.2
ndhF_p5	CGAGGTCAAGATCAACCTGG	TTCCCGGTTGTACTCGTAGT	55.0	50.0	58.0	58.1	2801 bp	0.2	47.2