Hotspot

trnR-trnT

IRa · 147679-148590 · 912 bp

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Pi 0.125

MarkerSeek score 83.80

Primer available yes

Label trnR-trnT

Feature Metadata

label_name: trnR-trnT
feature_type: igs
region: IRa
length_bp: 912
variable_sites: 232
pi: 0.125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnT_p1	GCGGGATTTGTCTCCATGAT	GTAGGCACACCAGACACAAA	50.0	50.0	58.0	58.0	1268 bp	0.2	47.5
trnR-trnT_p2	CCCTCGACTCCTTATCGACT	GTAGGCACACCAGACACAAA	55.0	50.0	58.0	58.0	1217 bp	0.2	47.5
trnR-trnT_p3	GCGGGATTTGTCTCCATGAT	GAACCCTTGGCCATAGTCAG	50.0	55.0	58.0	58.0	1506 bp	0.2	47.4
trnR-trnT_p4	TCCCTCGACTCCTTATCGAC	GTAGGCACACCAGACACAAA	55.0	50.0	58.0	58.0	1218 bp	0.2	47.4
trnR-trnT_p5	CCCTCGACTCCTTATCGACT	GAACCCTTGGCCATAGTCAG	55.0	55.0	58.0	58.0	1455 bp	0.2	47.4