Hotspot

trnT-trnR

IRb · 84261-85172 · 912 bp

Back to Adiantum

Pi 0.125

MarkerSeek score 83.80

Primer available yes

Label trnT-trnR

Feature Metadata

label_name: trnT-trnR
feature_type: igs
region: IRb
length_bp: 912
variable_sites: 232
pi: 0.125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnR_p1	GTAGGCACACCAGACACAAA	GCGGGATTTGTCTCCATGAT	50.0	50.0	58.0	58.0	1268 bp	0.2	47.5
trnT-trnR_p2	GTAGGCACACCAGACACAAA	CCCTCGACTCCTTATCGACT	50.0	55.0	58.0	58.0	1217 bp	0.2	47.5
trnT-trnR_p3	GAACCCTTGGCCATAGTCAG	GCGGGATTTGTCTCCATGAT	55.0	50.0	58.0	58.0	1506 bp	0.2	47.4
trnT-trnR_p4	GTAGGCACACCAGACACAAA	TCCCTCGACTCCTTATCGAC	50.0	55.0	58.0	58.0	1218 bp	0.2	47.4
trnT-trnR_p5	GAACCCTTGGCCATAGTCAG	CCCTCGACTCCTTATCGACT	55.0	55.0	58.0	58.0	1455 bp	0.2	47.4