Hotspot

rpl14-rpl16

LSC · 76736-77180 · 445 bp

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Pi 0.346

MarkerSeek score 76.60

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 259
pi: 0.346
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AAAACATGTTGGGTACGGCT	GAGACTTCCACATCCGGTTC	45.0	55.0	58.0	58.0	966-1072 bp	0.4	67.8
rpl14-rpl16_p2	AAACATGTTGGGTACGGCTT	GAGACTTCCACATCCGGTTC	45.0	55.0	58.0	58.0	965-1071 bp	0.4	67.8
rpl14-rpl16_p3	AAACATGTTGGGTACGGCTT	GCTGAGGCTGCTATGTTGAT	45.0	50.0	58.0	58.0	574-673 bp	0.4	67.6
rpl14-rpl16_p4	CCCCCTACGAGTGCATACTA	GAGACTTCCACATCCGGTTC	55.0	55.0	58.0	58.0	1122 bp	0.2	47.8
rpl14-rpl16_p5	CCCCCTACGAGTGCATACTA	GCTGAGGCTGCTATGTTGAT	55.0	50.0	58.0	58.0	724 bp	0.2	47.6