Hotspot

rpl20-rps12

LSC · 64017-64967 · 951 bp

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Pi 0.153

MarkerSeek score 84.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 283
pi: 0.153
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GCTGGTTTGCCGTTCTAAAC	CGGAAGAAGCCCAGGATTAC	50.0	55.0	58.0	58.0	1466-1493 bp	0.4	64.3
rpl20-rps12_p2	GCTGGTTTGCCGTTCTAAAC	GAAGAAGCCCAGGATTACGG	50.0	55.0	58.0	58.0	1464-1491 bp	0.4	64.3
rpl20-rps12_p3	GCTGGTTTGCCGTTCTAAAC	GGAAGAAGCCCAGGATTACG	50.0	55.0	58.0	58.0	1465-1492 bp	0.4	64.3
rpl20-rps12_p4	GCTGGTTTGCCGTTCTAAAC	GATTACGGTGTTGCGGATCT	50.0	50.0	58.0	58.1	1452-1479 bp	0.4	64.2
rpl20-rps12_p5	GCTGGTTTGCCGTTCTAAAC	GGGAGTGTGTACTAGGGTGT	50.0	55.0	58.0	58.1	1085-1089 bp	0.4	63.4