Hotspot

petA-psbJ

LSC · 58998-59740 · 743 bp

Back to Adiantum

Pi 0.247

MarkerSeek score 83.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 348
pi: 0.247
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGATGGGAGCAAGAGTAAC	AGGTTTGCCGGGTGTATTTT	55.0	45.0	58.1	58.0	1181-1516 bp	0.8	83.5
petA-psbJ_p2	GGAAAGAGGGAAAAGGTGGG	GACCGTTCATGTCTGGAGTC	55.0	55.0	58.1	58.0	1250-1610 bp	0.8	83.1
petA-psbJ_p3	CGGATGGGAGCAAGAGTAAC	GGCTGGCTGTCTGTAAGTAC	55.0	55.0	58.1	58.0	1786-2132 bp	0.8	83.0
petA-psbJ_p4	GGAAAGAGGGAAAAGGTGGG	AGGTTTGCCGGGTGTATTTT	55.0	45.0	58.1	58.0	1112-1447 bp	0.6	74.4
petA-psbJ_p5	CGGATGGGAGCAAGAGTAAC	GACCGTTCATGTCTGGAGTC	55.0	55.0	58.1	58.0	1319-1651 bp	0.6	74.3