Hotspot

rps4-trnL

LSC · 44040-44774 · 735 bp

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Pi 0.218

MarkerSeek score 84.60

Primer available yes

Label rps4-trnL

Feature Metadata

label_name: rps4-trnL
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 309
pi: 0.218
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL_p1	ACTTCGTTCCCACTGAGAGA	AGTCTCTGTACCTATCCCGC	50.0	55.0	58.0	58.0	1382 bp	0.2	47.8
rps4-trnL_p2	ACTTCGTTCCCACTGAGAGA	GTCTCTGTACCTATCCCGCT	50.0	55.0	58.0	58.0	1381 bp	0.2	47.8
rps4-trnL_p3	TTCGTTCCCACTGAGAGACT	AGTCTCTGTACCTATCCCGC	50.0	55.0	58.0	58.0	1380 bp	0.2	47.8
rps4-trnL_p4	TTCGTTCCCACTGAGAGACT	GTCTCTGTACCTATCCCGCT	50.0	55.0	58.0	58.0	1379 bp	0.2	47.8
rps4-trnL_p5	TCTGGTACTTCGTTCCCACT	GTCTCTGTACCTATCCCGCT	50.0	55.0	58.0	58.0	1387 bp	0.2	47.8