Hotspot

psbD-trnT

LSC · 33796-34944 · 1149 bp

Back to Adiantum

Pi 0.235

MarkerSeek score 83.20

Primer available yes

Label psbD-trnT

Feature Metadata

label_name: psbD-trnT
feature_type: igs
region: LSC
length_bp: 1149
variable_sites: 492
pi: 0.235
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT_p1	GGACCAGAGAAAGCTACTGC	CCCCATGGATGCAGCTTAAT	55.0	50.0	58.0	58.0	1766 bp	0.2	47.8
psbD-trnT_p2	CGGAGACAAAAACCGCAATC	CCCCATGGATGCAGCTTAAT	50.0	50.0	58.0	58.0	1786 bp	0.2	47.7
psbD-trnT_p3	GGAGACAAAAACCGCAATCG	CCCCATGGATGCAGCTTAAT	50.0	50.0	58.0	58.0	1785 bp	0.2	47.7
psbD-trnT_p4	GGACCAGAGAAAGCTACTGC	GTCTTGCCTGGAGTGTTGAA	55.0	50.0	58.0	58.0	2170 bp	0.2	47.6
psbD-trnT_p5	ACCACGAAGTCACAAAGGTC	CCCCATGGATGCAGCTTAAT	50.0	50.0	58.1	58.0	1495 bp	0.2	47.6