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psbM-petN

LSC · 28244-29156 · 913 bp

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Pi 0.282

MarkerSeek score 81.60

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 913
variable_sites: 456
pi: 0.282
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TCTTTACATTCAGACGGCCG	CGTTTTCCCTCTCGCTTGTA	50.0	50.0	58.0	57.9	955-994 bp	0.4	67.4
psbM-petN_p2	TCTTTACATTCAGACGGCCG	TACGTTTTCCCTCTCGCTTG	50.0	50.0	58.0	57.9	957-996 bp	0.4	67.4
psbM-petN_p3	TCTTTACATTCAGACGGCCG	CAAGCGGTAAGACGTAGGAC	50.0	55.0	58.0	58.1	1525-1548 bp	0.4	67.2
psbM-petN_p4	TCTCGATAGGACCGCTCAAT	CGTTTTCCCTCTCGCTTGTA	50.0	50.0	58.0	57.9	1117 bp	0.2	47.3
psbM-petN_p5	TCTCGATAGGACCGCTCAAT	TACGTTTTCCCTCTCGCTTG	50.0	50.0	58.0	57.9	1119 bp	0.2	47.3