Hotspot

rpoB-trnD

LSC · 26270-27213 · 944 bp

Back to Adiantum

Pi 0.314

MarkerSeek score 81.10

Primer available yes

Label rpoB-trnD

Feature Metadata

label_name: rpoB-trnD
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 513
pi: 0.314
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD_p1	TGGTATACGCGAGAATCCCT	ATTGTTACCCCTCCCGGTAA	50.0	50.0	58.0	58.0	1754 bp	0.2	47.7
rpoB-trnD_p2	TGGTATACGCGAGAATCCCT	CCGCTTCTAGCTAATCGGTC	50.0	55.0	58.0	57.9	2031 bp	0.2	47.5
rpoB-trnD_p3	TCCCTGATCTGAAATTGCGG	ATTGTTACCCCTCCCGGTAA	50.0	50.0	58.0	58.0	1777 bp	0.2	47.4
rpoB-trnD_p4	TGATCCGACTCCCGATTGTA	ATTGTTACCCCTCCCGGTAA	50.0	50.0	57.9	58.0	1731 bp	0.2	47.3
rpoB-trnD_p5	TGGTATACGCGAGAATCCCT	CGTTGCAATTTCCCGTATCG	50.0	50.0	58.0	57.9	1454 bp	0.2	47.2