Hotspot

matK-rps16

LSC · 2819-3620 · 802 bp

Back to Adiantum

Pi 0.249

MarkerSeek score 84.00

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 376
pi: 0.249
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GGTCACGTACGCTACCAATT	CTGAACGGGTGGCATCATAA	50.0	50.0	58.0	58.0	1709 bp	0.2	47.7
matK-rps16_p2	GGTCACGTACGCTACCAATT	CCACGTCGGATGTTACAAGT	50.0	50.0	58.0	57.9	1334 bp	0.2	47.4
matK-rps16_p3	GGTCACGTACGCTACCAATT	ACTCCTATCAGGCTACCACC	50.0	55.0	58.0	57.9	1384 bp	0.2	47.3
matK-rps16_p4	GGTCACGTACGCTACCAATT	CCGAAGCAATGTGGTAGGAA	50.0	50.0	58.0	57.9	1610 bp	0.2	47.1
matK-rps16_p5	GGTCACGTACGCTACCAATT	GGAAGCGGCGTCAAATTAAC	50.0	50.0	58.0	58.1	1031 bp	0.2	47.1