Hotspot

ycf3

LSC · 42244-44193 · 1950 bp

Back to Adenostyles

Pi 0.00154

MarkerSeek score 58.70

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1950
variable_sites: 3
pi: 0.00154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGGTTGGTTCTTCCCAAAGG	TCCCGCGAGAAGTAATGAGA	50.0	50.0	57.9	58.2	2107-2108 bp	1.0	77.6
ycf3_p2	TGGTTGGTTCTTCCCAAAGG	GGATCATTTTCCCGCGAGAA	50.0	50.0	57.9	58.3	2116-2117 bp	1.0	76.8
ycf3_p3	TGGTTGGTTCTTCCCAAAGG	ATCATTTTCCCGCGAGAAGT	50.0	45.0	57.9	57.6	2114-2115 bp	1.0	76.3
ycf3_p4	GGTTGGTTCTTCCCAAAGGA	TCCCGCGAGAAGTAATGAGA	50.0	50.0	57.6	58.2	2106-2107 bp	1.0	75.2
ycf3_p5	TGGTTGGTTCTTCCCAAAGG	CCCGCGAGAAGTAATGAGAA	50.0	50.0	57.9	57.4	2106-2107 bp	1.0	74.9