Hotspot

ycf1

IRb · 107352-107978 · 627 bp

Back to Adenostyles

Pi 0.00159
MarkerSeek score 59.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
627
variable_sites
1
pi
0.00159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGAAAAGAAAAAGGGAGGTTTGTG GCGGTGGTCGTATTTCTTCT 37.5 50.0 58.0 58.0 710 bp 1.0 47.9
ycf1_p2 AAGAAAAAGGGAGGTTTGTG GCGGTGGTCGTATTTCTTCT 40.0 50.0 53.9 58.0 706 bp 1.0 47.1
ycf1_p3 AAAGAAAAAGGGAGGTTTGTG GCGGTGGTCGTATTTCTTCT 38.1 50.0 54.8 58.0 707 bp 1.0 46.1
ycf1_p4 AGAAAAGAAAAAGGGAGGTTTGT GCGGTGGTCGTATTTCTTCT 34.8 50.0 56.7 58.0 710 bp 1.0 45.8
ycf1_p5 GAAAAGAAAAAGGGAGGTTTGTG GCGGTGGTCGTATTTCTTCT 39.1 50.0 56.7 58.0 709 bp 1.0 45.6