Hotspot

rps16

LSC · 5277-6372 · 1096 bp

Back to Adenostyles

Pi 0.00183

MarkerSeek score 53.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1096
variable_sites: 2
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAATAGAGCAAGAAAAGAAGGA	CTGTGTCTATGTACCGAGCG	34.8	55.0	55.6	57.9	1288-1289 bp	1.0	40.2
rps16_p2	AGAGCAAGAAAAGAAGGAAA	CTGTGTCTATGTACCGAGCG	35.0	55.0	52.6	57.9	1283-1284 bp	1.0	40.2
rps16_p3	TGAATAGAGCAAGAAAAGAAGGA	GTCTATGTACCGAGCGAACC	34.8	55.0	55.6	57.9	1284-1285 bp	1.0	40.2
rps16_p4	AGAGCAAGAAAAGAAGGAAA	GTCTATGTACCGAGCGAACC	35.0	55.0	52.6	57.9	1279-1280 bp	1.0	40.2
rps16_p5	TGAATAGAGCAAGAAAAGAAGGA	CCGAGCGAACCAATGACTAT	34.8	50.0	55.6	57.8	1275-1276 bp	1.0	40.2