Hotspot

ndhF-rpl32

SSC · 110178-111263 · 1086 bp

Back to Adenostyles

Pi 0.0037
MarkerSeek score 61.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1086
variable_sites
4
pi
0.0037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG CGCTGCCCAATATCCTTTCT 50.0 50.0 58.0 58.0 1262-1268 bp 1.0 80.2
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA CGCTGCCCAATATCCTTTCT 45.0 50.0 58.0 58.0 1260-1266 bp 1.0 80.1
ndhF-rpl32_p3 TTGCCGTCGGAAAAAGAAGA CGCTGCCCAATATCCTTTCT 45.0 50.0 58.0 58.0 1261-1267 bp 1.0 80.1
ndhF-rpl32_p4 GTTGCCGTCGGAAAAAGAAG ACAAAGCTTTTAACGCTGCC 50.0 45.0 58.0 57.9 1275-1281 bp 1.0 79.3
ndhF-rpl32_p5 GTTGCCGTCGGAAAAAGAAG AACGCTGCCCAATATCCTTT 50.0 45.0 58.0 57.9 1264-1270 bp 1.0 79.2