Hotspot

rpl14-rpl16

LSC · 80085-80199 · 115 bp

Back to Adenostyles

Pi 0.0174

MarkerSeek score 62.50

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 2
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCGACGATTGCTAGCTCCTA	AATAGCAGCGTCCAAAATGC	50.0	45.0	58.0	57.4	260 bp	1.0	75.8
rpl14-rpl16_p2	TCGACGATTGCTAGCTCCTA	GCGTCCAAAATGCCTATACG	50.0	50.0	58.0	57.3	253 bp	1.0	75.0
rpl14-rpl16_p3	CCCGCTGTTATCTGCTACAT	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.5	57.4	212 bp	1.0	72.0
rpl14-rpl16_p4	CACATCAATTTTCGAGCCCC	AATAGCAGCGTCCAAAATGC	50.0	45.0	57.4	57.4	229 bp	1.0	71.6
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	GCGTCCAAAATGCCTATACG	50.0	50.0	57.5	57.3	205 bp	1.0	71.2