Hotspot

atpB-rbcL

LSC · 54070-54804 · 735 bp

Back to Adenostyles

Pi 0.00136

MarkerSeek score 59.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 1
pi: 0.00136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATACGCCCCAGGTTTGTTTT	GCTTTGAATCCAACACTCGC	45.0	50.0	58.0	58.0	847-850 bp	1.0	79.8
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.0	58.0	891-894 bp	1.0	79.6
atpB-rbcL_p3	GCGTGGTAACCCCAGAATTA	GCTTTGAATCCAACACTCGC	50.0	50.0	57.6	58.0	822-825 bp	1.0	76.6
atpB-rbcL_p4	GATACGCCCCAGGTTTGTTT	GCTTTGAATCCAACACTCGC	50.0	50.0	58.5	58.0	848-851 bp	1.0	76.2
atpB-rbcL_p5	CATTTTGCCTGGCGGAAAAG	GCTTTGAATCCAACACTCGC	50.0	50.0	58.6	58.0	899-902 bp	1.0	75.3