Hotspot

trnG-UCC-psbD

LSC · 31082-32564 · 1483 bp

Back to Adenostyles

Pi 0.0027

MarkerSeek score 60.50

Primer available yes

Label trnG-UCC-psbD

Feature Metadata

label_name: trnG-UCC-psbD
feature_type: igs
region: LSC
length_bp: 1483
variable_sites: 4
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-psbD_p1	AATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1641-1644 bp	1.0	72.5
trnG-UCC-psbD_p2	ATCAAACCGAAAGACCCCTT	GAAACGGTCCCTACGTAACC	45.0	55.0	57.0	58.0	1640-1643 bp	1.0	72.5
trnG-UCC-psbD_p3	AATCAAACCGAAAGACCCCT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1659-1662 bp	1.0	71.7
trnG-UCC-psbD_p4	ATCAAACCGAAAGACCCCTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.0	58.1	1658-1661 bp	1.0	71.7
trnG-UCC-psbD_p5	GAATCAAACCGAAAGACCCCT	GAAACGGTCCCTACGTAACC	47.6	55.0	58.2	58.0	1642-1645 bp	1.0	70.6