Hotspot

atpI-atpH

LSC · 25270-26385 · 1116 bp

Back to Adenostyles

Pi 0.0018

MarkerSeek score 60.00

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 2
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTTGCAACTTTAGCCGCG	ATAACGGAAGCGGCAGAAAT	45.0	45.0	58.5	58.0	1202-1204 bp	1.0	75.8
atpI-atpH_p2	TTTTTGCAACTTTAGCCGCG	TACCTTGACCAACTCCAGGT	45.0	50.0	58.5	57.9	1258-1260 bp	1.0	75.2
atpI-atpH_p3	TTTTTGCAACTTTAGCCGCG	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.5	57.9	1218-1220 bp	1.0	74.9
atpI-atpH_p4	TCAGTGGTTCCTATCCCTGT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.4	58.0	1263-1265 bp	1.0	74.8
atpI-atpH_p5	TTTTTGCAACTTTAGCCGCG	AATAACGGAAGCGGCAGAAA	45.0	45.0	58.5	58.2	1203-1205 bp	1.0	74.7