Hotspot

ndhI

SSC · 115616-116116 · 501 bp

Back to Adenostemma

Pi 0.446

MarkerSeek score 78.10

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 185
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGCCGAGTGTGAACGATTAC	AAAAACAATTCTCGCCCCCT	50.0	45.0	58.0	58.0	1105 bp	0.5	59.9
ndhI_p2	TACATATCCGTTCCCGAGCT	AAAAACAATTCTCGCCCCCT	50.0	45.0	58.0	58.0	921 bp	0.5	59.9
ndhI_p3	TTTGTGACCTTTGAGGAGCC	AAAAACAATTCTCGCCCCCT	50.0	45.0	58.0	58.0	1311 bp	0.5	59.8
ndhI_p4	GACCTTTGAGGAGCCGTATG	AAAAACAATTCTCGCCCCCT	55.0	45.0	58.1	58.0	1306 bp	0.5	59.5
ndhI_p5	GTACATATCCGTTCCCGAGC	AAAAACAATTCTCGCCCCCT	55.0	45.0	57.9	58.0	922 bp	0.5	59.4