Hotspot

ndhG

SSC · 116477-117007 · 531 bp

Back to Adenostemma

Pi 0.477

MarkerSeek score 80.50

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 226
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AGGGGGCGAGAATTGTTTTT	TGAATCGATGAGTTCCCCCA	45.0	50.0	58.0	58.4	1488 bp	0.5	56.6
ndhG_p2	AGGGGGCGAGAATTGTTTTT	GAGTCCAATAGCGGCTTCAG	45.0	55.0	58.0	58.4	1259 bp	0.5	56.5
ndhG_p3	AGGGGGCGAGAATTGTTTTT	AATAGCGAGTCCAATAGCGG	45.0	50.0	58.0	57.6	1265 bp	0.5	56.4
ndhG_p4	TTGATCACATCCGAGCGTTT	TGAATCGATGAGTTCCCCCA	45.0	50.0	57.9	58.4	1933 bp	0.5	55.9
ndhG_p5	TTGATCACATCCGAGCGTTT	GAGTCCAATAGCGGCTTCAG	45.0	55.0	57.9	58.4	1704 bp	0.5	55.9