Hotspot

ccsA

SSC · 119906-120874 · 969 bp

Back to Adenostemma

Pi 0.475

MarkerSeek score 80.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 383
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTTTTTGATTCTGGACCGCG	GTGAAATCGGTAGACACGCT	50.0	50.0	58.0	58.0	1581 bp	0.5	59.6
ccsA_p2	GTTTTTGATTCTGGACCGCG	TGAAATCGGTAGACACGCTG	50.0	50.0	58.0	58.0	1580 bp	0.5	59.6
ccsA_p3	GTTTTTGATTCTGGACCGCG	AATTTTGGTGGTGGGGAGTC	50.0	50.0	58.0	58.0	2104 bp	0.5	59.6
ccsA_p4	TTTACGAGAACCGCGTGAAT	GTGAAATCGGTAGACACGCT	45.0	50.0	57.9	58.0	1285 bp	0.5	59.4
ccsA_p5	TTTACGAGAACCGCGTGAAT	TGAAATCGGTAGACACGCTG	45.0	50.0	57.9	58.0	1284 bp	0.5	59.4