Hotspot

rpl32-ndhF

SSC · 121985-122852 · 868 bp

Back to Adenostemma

Pi 0.426

MarkerSeek score 82.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 868
variable_sites: 356
pi: 0.426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GACTCCCCACCACCAAAATT	GCAGATTTAGCAATCGCACC	50.0	50.0	58.0	57.9	2005 bp	0.5	59.0
rpl32-ndhF_p2	GACTCCCCACCACCAAAATT	AACAGGTCGTGTGAACCAAA	50.0	45.0	58.0	57.9	1790 bp	0.5	59.0
rpl32-ndhF_p3	GACTCCCCACCACCAAAATT	ACAGGTCGTGTGAACCAAAA	50.0	45.0	58.0	57.9	1789 bp	0.5	59.0
rpl32-ndhF_p4	AATAAGACTCCCCACCACCA	GCAGATTTAGCAATCGCACC	50.0	50.0	57.9	57.9	2010 bp	0.5	58.7
rpl32-ndhF_p5	ATAAGACTCCCCACCACCAA	GCAGATTTAGCAATCGCACC	50.0	50.0	57.9	57.9	2009 bp	0.5	58.7