Hotspot

ndhE-psaC

SSC · 117530-117769 · 240 bp

Back to Adenostemma

Pi 0.431

MarkerSeek score 75.20

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 90
pi: 0.431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	TAATCGGCGCAATTGCTGTA	TCCTCTGTTCTTGGAGCAGA	45.0	50.0	58.3	58.0	934 bp	0.5	57.1
ndhE-psaC_p2	CTGAAGCCGCTATTGGACTC	TCCTCTGTTCTTGGAGCAGA	55.0	50.0	58.4	58.0	472 bp	0.5	56.5
ndhE-psaC_p3	CCGCTATTGGACTCGCTATT	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	57.6	58.0	466 bp	0.5	56.4
ndhE-psaC_p4	TAATCGGCGCAATTGCTGTA	ACATCCGTCCCAAGGTATCA	45.0	50.0	58.3	58.1	896 bp	0.5	56.3
ndhE-psaC_p5	TAATCGGCGCAATTGCTGTA	CAACCGACACAATCCTCTGT	45.0	50.0	58.3	57.8	946 bp	0.5	55.9