Hotspot

ndhG-ndhE

SSC · 117008-117223 · 216 bp

Back to Adenostemma

Pi 0.455

MarkerSeek score 75.00

Primer available yes

Label ndhG-ndhE

Feature Metadata

label_name: ndhG-ndhE
feature_type: igs
region: SSC
length_bp: 216
variable_sites: 85
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhE_p1	AGTCACTTCCGTGGTCTGTA	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.0	58.0	1140 bp	0.5	59.8
ndhG-ndhE_p2	GTCACTTCCGTGGTCTGTAC	TCCTCTGTTCTTGGAGCAGA	55.0	50.0	57.9	58.0	1139 bp	0.5	59.4
ndhG-ndhE_p3	AACTCCCCTTTTGTAGCTGC	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.1	58.0	1274 bp	0.5	59.2
ndhG-ndhE_p4	CATCTTTGGACCGTGGGAAA	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.1	58.0	1163 bp	0.5	59.1
ndhG-ndhE_p5	TCTTTGGACCGTGGGAAATG	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.1	58.0	1161 bp	0.5	59.1