Hotspot

ndhI-ndhG

SSC · 116117-116476 · 360 bp

Back to Adenostemma

Pi 0.478

MarkerSeek score 77.20

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 360
variable_sites: 155
pi: 0.478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	AACAAACAGTACGAGCTGCA	GCAGCTACAAAAGGGGAGTT	45.0	50.0	58.1	58.1	1003 bp	0.5	58.9
ndhI-ndhG_p2	ACAAACAGTACGAGCTGCAA	GCAGCTACAAAAGGGGAGTT	45.0	50.0	58.1	58.1	1002 bp	0.5	58.9
ndhI-ndhG_p3	AACAAACAGTACGAGCTGCA	TTTCCCACGGTCCAAAGATG	45.0	50.0	58.1	58.1	1114 bp	0.5	58.8
ndhI-ndhG_p4	ACAAACAGTACGAGCTGCAA	CATTTCCCACGGTCCAAAGA	45.0	50.0	58.1	58.1	1115 bp	0.5	58.8
ndhI-ndhG_p5	ACAAACAGTACGAGCTGCAA	TTTCCCACGGTCCAAAGATG	45.0	50.0	58.1	58.1	1113 bp	0.5	58.8