Hotspot

ycf1-rps15

SSC · 111448-111841 · 394 bp

Back to Adenostemma

Pi 0.446

MarkerSeek score 77.40

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 394
variable_sites: 154
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AATTATGGAACCTGCCCGTC	CCGCAGACCTCTCTGAGATA	50.0	55.0	57.9	58.0	1064 bp	0.5	59.4
ycf1-rps15_p2	AATTATGGAACCTGCCCGTC	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	1338 bp	0.5	59.1
ycf1-rps15_p3	AATTATGGAACCTGCCCGTC	TAAGCCAGTAACCGTTGACG	50.0	50.0	57.9	57.9	1099 bp	0.5	59.0
ycf1-rps15_p4	TATGGAACCTGCCCGTCTAT	CCGCAGACCTCTCTGAGATA	50.0	55.0	57.9	58.0	1061 bp	0.5	59.0
ycf1-rps15_p5	TATGGAACCTGCCCGTCTAT	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	1335 bp	0.5	58.8