Hotspot

ndhH

SSC · 115533-116714 · 1182 bp

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Pi 0.481

MarkerSeek score 79.90

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 464
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GGTTTGCGGAAAATTCTGGG	TCACTGCTAAGGATGGACCA	50.0	50.0	57.9	58.0	1719 bp	0.5	59.0
ndhH_p2	GGTTTGCGGAAAATTCTGGG	TGCTAAGGATGGACCAATGC	50.0	50.0	57.9	57.9	1715 bp	0.5	58.9
ndhH_p3	GGTTTGCGGAAAATTCTGGG	AAGAATTCCCAAAGGCCCAG	50.0	50.0	57.9	58.1	1619 bp	0.5	58.9
ndhH_p4	GGTTTGCGGAAAATTCTGGG	GAAGAATTCCCAAAGGCCCA	50.0	50.0	57.9	58.1	1620 bp	0.5	58.9
ndhH_p5	GGTTTGCGGAAAATTCTGGG	TGAAGAATTCCCAAAGGCCC	50.0	50.0	57.9	58.1	1621 bp	0.5	58.9