Hotspot

ndhD

SSC · 121480-123009 · 1530 bp

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Pi 0.464

MarkerSeek score 82.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 639
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GATACCCTGGGACGGATGTA	GAGCTGGGACCCAAAAGAAA	55.0	50.0	58.0	58.0	2226 bp	0.5	59.9
ndhD_p2	GATACCCTGGGACGGATGTA	AACTAGTGAGAACCCGGACA	55.0	50.0	58.0	58.0	1963 bp	0.5	59.9
ndhD_p3	GATACCCTGGGACGGATGTA	ACTAGTGAGAACCCGGACAA	55.0	50.0	58.0	58.0	1962 bp	0.5	59.9
ndhD_p4	CGCTCCTAAACCAATTCGGA	GAGCTGGGACCCAAAAGAAA	50.0	50.0	58.0	58.0	2441 bp	0.5	59.8
ndhD_p5	TCGCTCCTAAACCAATTCGG	GAGCTGGGACCCAAAAGAAA	50.0	50.0	58.0	58.0	2442 bp	0.5	59.8