Hotspot

ndhA

SSC · 116716-118879 · 2164 bp

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Pi 0.465

MarkerSeek score 80.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2164
variable_sites: 902
pi: 0.465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGCAAAAAGAGGGGGATTCA	TCAAAGTGTATTCGGCCTCG	50.0	50.0	58.1	58.0	2847 bp	0.5	59.5
ndhA_p2	TGGCAAAAAGAGGGGGATTC	TCAAAGTGTATTCGGCCTCG	50.0	50.0	58.1	58.0	2848 bp	0.5	59.5
ndhA_p3	GGCAAAAAGAGGGGGATTCA	GCGTGGGACAAGGTTATCAT	50.0	50.0	58.1	58.0	2766 bp	0.5	59.2
ndhA_p4	TGGCAAAAAGAGGGGGATTC	GCGTGGGACAAGGTTATCAT	50.0	50.0	58.1	58.0	2767 bp	0.5	59.2
ndhA_p5	AGTCGAAGCACCAAAAGGAG	TCAAAGTGTATTCGGCCTCG	50.0	50.0	58.1	58.0	2612 bp	0.5	59.0