Hotspot

ccsA-trnL-UAG

SSC · 124210-124318 · 109 bp

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Pi 0.408

MarkerSeek score 76.00

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 109
variable_sites: 40
pi: 0.408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	TGATCCACATAAAAGGGCCG	CTCGCCATCAATCGACTCAA	50.0	50.0	57.9	58.1	1114 bp	0.5	59.0
ccsA-trnL-UAG_p2	TGATCCACATAAAAGGGCCG	GAGTTCAAGACAAGCCGCTA	50.0	50.0	57.9	57.9	664 bp	0.5	58.9
ccsA-trnL-UAG_p3	AACCACTCGGATTGAAGAGC	CTCGCCATCAATCGACTCAA	50.0	50.0	57.9	58.1	1059 bp	0.5	58.6
ccsA-trnL-UAG_p4	TGATCCACATAAAAGGGCCG	TAGACACGCTGCTCTTAGGA	50.0	50.0	57.9	57.9	632 bp	0.5	58.6
ccsA-trnL-UAG_p5	TGATCCACATAAAAGGGCCG	ACAAGCCGCTATGGTGAAAT	50.0	45.0	57.9	57.9	655 bp	0.5	58.6