Hotspot

ycf1

Genome · 155604-162143 · 6540 bp

Back to Adenophora

Pi 0.0204

MarkerSeek score 57.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 6540
variable_sites: 421
pi: 0.0204
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATACTGAAACGGCTGCCATT	CTTCTCCCAAGCCAACAACT	45.0	50.0	57.9	58.0	893-1109 bp	1.0	88.7
ycf1_p2	CCTCTTTAGGCGTTCTTGCT	CTTCTCCCAAGCCAACAACT	50.0	50.0	57.9	58.0	474 bp	0.1	43.1
ycf1_p3	CCTCTTTAGGCGTTCTTGCT	ACGAATCAAGGAAGACGTCG	50.0	50.0	57.9	58.0	622 bp	0.1	43.0
ycf1_p4	CCTCTTTAGGCGTTCTTGCT	GAGAGAATGGAACTGCCCAG	50.0	55.0	57.9	58.0	174 bp	0.1	42.9
ycf1_p5	CCTCTTTAGGCGTTCTTGCT	CATGGAGGAATTGGAGACCG	50.0	55.0	57.9	58.0	592 bp	0.1	42.9