Hotspot

ndhE-ndhF

Genome · 140519-140624 · 106 bp

Back to Adenophora

Pi 0.0352

MarkerSeek score 60.20

Primer available yes

Label ndhE-ndhF

Feature Metadata

label_name: ndhE-ndhF
feature_type: igs
region: Genome
length_bp: 106
variable_sites: 7
pi: 0.0352
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhF_p1	CGGATTGATCACGAGTCGAA	CGGGCGCATTTCATCTTATC	50.0	50.0	57.8	57.4	290-291 bp	0.2	41.7
ndhE-ndhF_p2	CGGATTGATCACGAGTCGAA	TCAAATTTATCGGTGGCGGG	50.0	50.0	57.8	58.6	306-307 bp	0.2	41.6
ndhE-ndhF_p3	TACGGATTGATCACGAGTCG	CGGGCGCATTTCATCTTATC	50.0	50.0	57.2	57.4	292-293 bp	0.2	37.2
ndhE-ndhF_p4	TACGGATTGATCACGAGTCG	TCAAATTTATCGGTGGCGGG	50.0	50.0	57.2	58.6	308-309 bp	0.2	37.1
ndhE-ndhF_p5	ACGGATTGATCACGAGTCGA	CGGGCGCATTTCATCTTATC	50.0	50.0	58.9	57.4	291-292 bp	0.2	35.9