Hotspot

ndhB-trnI-CAU

Genome · 103225-105621 · 2397 bp

Back to Adenophora

Pi 0.0576
MarkerSeek score 84.50
Primer available yes
Label ndhB-trnI-CAU

Feature Metadata

label_name
ndhB-trnI-CAU
feature_type
igs
region
Genome
length_bp
2397
variable_sites
324
pi
0.0576
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.72
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnI-CAU_p1 CCAGGAATATCAATGAACCCGA TCCTGTTAAGCATCCATGGC 45.5 50.0 57.9 57.9 1505-2534 bp 0.9 70.9
ndhB-trnI-CAU_p2 CCAGGAATATCAATGAACCCGA CCTGTTAAGCATCCATGGCT 45.5 50.0 57.9 57.9 1504-2533 bp 0.9 70.8
ndhB-trnI-CAU_p3 TCAATTGCTCAGGATACCCT TCCTGTTAAGCATCCATGGC 45.0 50.0 55.9 57.9 1478-2507 bp 0.9 70.7
ndhB-trnI-CAU_p4 TCAATTGCTCAGGATACCCT CCTGTTAAGCATCCATGGCT 45.0 50.0 55.9 57.9 1477-2506 bp 0.9 70.7
ndhB-trnI-CAU_p5 ACCAGGAATATCAATGAACCCG TCCTGTTAAGCATCCATGGC 45.5 50.0 58.2 57.9 1506-2535 bp 0.9 70.1