Hotspot

trnT-UGU-psbJ

Genome · 44738-47663 · 2926 bp

Back to Adenophora

Pi 0.0385

MarkerSeek score 80.50

Primer available yes

Label trnT-UGU-psbJ

Feature Metadata

label_name: trnT-UGU-psbJ
feature_type: igs
region: Genome
length_bp: 2926
variable_sites: 301
pi: 0.0385
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-psbJ_p1	GATGGTCATCGGTTCGAGTC	ATTCTGGATTGGGTTCATCC	55.0	45.0	58.2	55.1	900-2984 bp	0.4	40.4
trnT-UGU-psbJ_p2	GATGGTCATCGGTTCGAGTC	TCCTATTCTGGATTGGGTTCA	55.0	42.9	58.2	56.0	904-2988 bp	0.4	40.2
trnT-UGU-psbJ_p3	GATGGTCATCGGTTCGAGTC	AGTTCCTATTCTGGATTGGGT	55.0	42.9	58.2	56.0	907-2991 bp	0.4	40.1
trnT-UGU-psbJ_p4	GATGGTCATCGGTTCGAGTC	AGTTCCTATTCTGGATTGGGTTC	55.0	43.5	58.2	57.9	907-2991 bp	0.4	39.1
trnT-UGU-psbJ_p5	GATGGTCATCGGTTCGAGTC	GTTCCTATTCTGGATTGGGTTCA	55.0	43.5	58.2	58.1	906-2990 bp	0.4	38.9