Hotspot

petG-petL

Genome · 35806-35996 · 191 bp

Back to Adenophora

Pi 0.02

MarkerSeek score 61.00

Primer available yes

Label petG-petL

Feature Metadata

label_name: petG-petL
feature_type: igs
region: Genome
length_bp: 191
variable_sites: 11
pi: 0.02
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-petL_p1	TCAAAGGTCCAACTGATCGC	TAGCTCCTTCATGCCTACGA	50.0	50.0	58.2	57.9	396-411 bp	1.0	83.3
petG-petL_p2	TCAAAGGTCCAACTGATCGC	GGGTTTCTACTAGCGGCTTT	50.0	50.0	58.2	57.6	362-377 bp	1.0	81.2
petG-petL_p3	TCAAAGGTCCAACTGATCGC	ATCGGGCTGAGCAAAATACG	50.0	50.0	58.2	58.7	320-335 bp	1.0	79.3
petG-petL_p4	TCAAAGGTCCAACTGATCGC	AGCTCCTTCATGCCTACGAT	50.0	50.0	58.2	58.6	395-410 bp	1.0	79.2
petG-petL_p5	TCAAAGGTCCAACTGATCGC	TTTCGGGTTTCTACTAGCGG	50.0	50.0	58.2	57.3	366-381 bp	1.0	79.0