Hotspot

psbB-rpl20

Genome · 29747-33024 · 3278 bp

Back to Adenophora

Pi 0.0797

MarkerSeek score 74.30

Primer available yes

Label psbB-rpl20

Feature Metadata

label_name: psbB-rpl20
feature_type: igs
region: Genome
length_bp: 3278
variable_sites: 669
pi: 0.0797
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-rpl20_p1	GTGAAATCGGTAGACACGCT	TGCGTCGATTAAAAGCTCGA	50.0	45.0	58.0	58.0	1493-1495 bp	0.2	56.4
psbB-rpl20_p2	TGAAATCGGTAGACACGCTG	TGCGTCGATTAAAAGCTCGA	50.0	45.0	58.0	58.0	1492-1494 bp	0.2	56.4
psbB-rpl20_p3	GTGAAATCGGTAGACACGCT	CGACAGCGTCTTGATGAAGA	50.0	50.0	58.0	58.0	1317 bp	0.1	43.8
psbB-rpl20_p4	GTGAAATCGGTAGACACGCT	CTCGACAGCGTCTTGATGAA	50.0	50.0	58.0	58.0	1319 bp	0.1	43.8
psbB-rpl20_p5	GTGAAATCGGTAGACACGCT	TCGACAGCGTCTTGATGAAG	50.0	50.0	58.0	58.0	1318 bp	0.1	43.8