primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rpoA-petD_p1	rpoA-petD	1	AGTTCGGGATTTATTTACCGCA	CCCATTTCGTCGTCCAGTAG	22	20	40.9	55.0	58.1	58.1	0.0	0.0	2.201089	23200	24383	1334-1349	0.900000	67.1
rpoA-petD_p2	rpoA-petD	2	AGTTCGGGATTTATTTACCGCA	TTGGTTGGGTATTGGTGCAA	22	20	40.9	45.0	58.1	57.9	0.0	0.0	2.211763	23200	24383	1277-1292	0.900000	67.1
rpoA-petD_p3	rpoA-petD	3	AGTTCGGGATTTATTTACCGCA	TTTGGTTGGGTATTGGTGCA	22	20	40.9	45.0	58.1	57.9	0.0	0.0	2.211763	23200	24383	1278-1293	0.900000	67.1
rpoA-petD_p4	rpoA-petD	4	AGTTCGGGATTTATTTACCGCA	CAGTAGCGACAACCGTCTTT	22	20	40.9	50.0	58.1	58.2	0.0	0.0	2.344090	23200	24383	1320-1335	0.900000	66.0
rpoA-petD_p5	rpoA-petD	5	GTTCGGGATTTATTTACCGCA	CCCATTTCGTCGTCCAGTAG	21	20	42.9	55.0	56.7	58.1	0.0	0.0	2.391027	23200	24383	1333-1348	0.900000	65.6
psbT-psbB_p1	psbT-psbB	1	GAACTTTTGGCGGCTCTCTA	GCTCAAGTCGAATTTGGAGC	20	20	50.0	50.0	57.9	57.5	11.4	18.2	0.628994	27992	28219	360-383	1.000000	82.1
psbT-psbB_p2	psbT-psbB	2	GAACTTTTGGCGGCTCTCTA	GGTGCTAGAACCCTCTTCAG	20	20	50.0	55.0	57.9	57.3	11.4	9.0	0.776918	27992	28219	414-437	1.000000	80.6
psbT-psbB_p3	psbT-psbB	3	AACTTTTGGCGGCTCTCTAA	GCTCAAGTCGAATTTGGAGC	20	20	45.0	50.0	57.4	57.5	11.4	18.2	1.095710	27992	28219	359-382	1.000000	78.4
psbT-psbB_p4	psbT-psbB	4	ACTTTTGGCGGCTCTCTAAA	GCTCAAGTCGAATTTGGAGC	20	20	45.0	50.0	57.4	57.5	11.4	18.2	1.095710	27992	28219	358-381	1.000000	78.4
psbT-psbB_p5	psbT-psbB	5	ACTTTTGGCGGCTCTCTAAA	GGTGCTAGAACCCTCTTCAG	20	20	45.0	55.0	57.4	57.3	11.4	9.0	1.243633	27992	28219	412-435	1.000000	76.8
psbB-rpl20_p1	psbB-rpl20	1	GTGAAATCGGTAGACACGCT	TGCGTCGATTAAAAGCTCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.022496	31384	31533	1493-1495	0.200000	56.4
psbB-rpl20_p2	psbB-rpl20	2	TGAAATCGGTAGACACGCTG	TGCGTCGATTAAAAGCTCGA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023867	31384	31533	1492-1494	0.200000	56.4
psbB-rpl20_p3	psbB-rpl20	3	GTGAAATCGGTAGACACGCT	CGACAGCGTCTTGATGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.025218	31384	31533	1317-1317	0.100000	43.8
psbB-rpl20_p4	psbB-rpl20	4	GTGAAATCGGTAGACACGCT	CTCGACAGCGTCTTGATGAA	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.025218	31384	31533	1319-1319	0.100000	43.8
psbB-rpl20_p5	psbB-rpl20	5	GTGAAATCGGTAGACACGCT	TCGACAGCGTCTTGATGAAG	20	20	50.0	50.0	58.0	58.0	0.0	7.0	0.025218	31384	31533	1318-1318	0.100000	43.8
petG-petL_p1	petG-petL	1	TCAAAGGTCCAACTGATCGC	TAGCTCCTTCATGCCTACGA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.258955	35806	35996	396-411	1.000000	83.3
petG-petL_p2	petG-petL	2	TCAAAGGTCCAACTGATCGC	GGGTTTCTACTAGCGGCTTT	20	20	50.0	50.0	58.2	57.6	0.0	8.3	0.589841	35806	35996	362-377	1.000000	81.2
petG-petL_p3	petG-petL	3	TCAAAGGTCCAACTGATCGC	ATCGGGCTGAGCAAAATACG	20	20	50.0	50.0	58.2	58.7	0.0	6.8	0.892048	35806	35996	320-335	1.000000	79.3
petG-petL_p4	petG-petL	4	TCAAAGGTCCAACTGATCGC	AGCTCCTTCATGCCTACGAT	20	20	50.0	50.0	58.2	58.6	0.0	0.0	0.773256	35806	35996	395-410	1.000000	79.2
petG-petL_p5	petG-petL	5	TCAAAGGTCCAACTGATCGC	TTTCGGGTTTCTACTAGCGG	20	20	50.0	50.0	58.2	57.3	0.0	0.0	0.851778	35806	35996	366-381	1.000000	79.0
psbJ-ndhC_p1	psbJ-ndhC	1	ATTCCGGATTGGGTTCATCC	GGGGCTAATACTCCCGAAAG	20	20	50.0	55.0	57.6	57.5	4.1	0.0	0.910510	37812	40633	1412-2946	0.300000	56.7
psbJ-ndhC_p2	psbJ-ndhC	2	ATTCCGGATTGGGTTCATCC	GGGGGCTAATACTCCCGAAA	20	20	50.0	55.0	57.6	58.9	4.1	0.0	1.230744	37812	40633	1413-2947	0.300000	54.2
psbJ-ndhC_p3	psbJ-ndhC	3	ATTCCGGATTGGGTTCATCC	AATGGGGGCTAATACTCCCG	20	20	50.0	55.0	57.6	58.9	4.1	0.0	1.300148	37812	40633	1416-2950	0.300000	53.6
psbJ-ndhC_p4	psbJ-ndhC	4	ATTCCGGATTGGGTTCATCC	GGGGGCTAATACTCCCGAA	20	19	50.0	57.9	57.6	58.2	4.1	0.0	1.520578	37812	40633	1413-2947	0.300000	51.8
psbJ-ndhC_p5	psbJ-ndhC	5	ATTCCGGATTGGGTTCATCC	ATGGGGGCTAATACTCCCG	20	19	50.0	57.9	57.6	58.2	4.1	0.0	1.591515	37812	40633	1415-2949	0.300000	51.3
trnT-UGU-psbJ_p1	trnT-UGU-psbJ	1	GATGGTCATCGGTTCGAGTC	ATTCTGGATTGGGTTCATCC	20	20	55.0	45.0	58.2	55.1	0.0	0.0	3.105499	44738	47663	900-2984	0.400000	40.4
trnT-UGU-psbJ_p2	trnT-UGU-psbJ	2	GATGGTCATCGGTTCGAGTC	TCCTATTCTGGATTGGGTTCA	20	21	55.0	42.9	58.2	56.0	0.0	0.0	3.131808	44738	47663	904-2988	0.400000	40.2
trnT-UGU-psbJ_p3	trnT-UGU-psbJ	3	GATGGTCATCGGTTCGAGTC	AGTTCCTATTCTGGATTGGGT	20	21	55.0	42.9	58.2	56.0	0.0	0.0	3.145092	44738	47663	907-2991	0.400000	40.1
trnT-UGU-psbJ_p4	trnT-UGU-psbJ	4	GATGGTCATCGGTTCGAGTC	AGTTCCTATTCTGGATTGGGTTC	20	23	55.0	43.5	58.2	57.9	0.0	0.0	3.275956	44738	47663	907-2991	0.400000	39.1
trnT-UGU-psbJ_p5	trnT-UGU-psbJ	5	GATGGTCATCGGTTCGAGTC	GTTCCTATTCTGGATTGGGTTCA	20	23	55.0	43.5	58.2	58.1	0.0	0.0	3.303210	44738	47663	906-2990	0.400000	38.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTTCCCCAGTTCAAGCCTG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.447937	62866	64957	1755-2741	0.800000	80.4
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTTTCCCCAGTTCAAGCCT	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.621405	62866	64957	1756-2742	0.800000	79.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTTCCCCAGTTCAAGCCTG	AGAGACCACTTCTTCCCCAT	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.647452	62866	64957	1816-2802	0.800000	78.8
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTTTCCCCAGTTCAAGCCT	AGAGACCACTTCTTCCCCAT	20	20	45.0	50.0	57.5	57.6	0.0	0.0	0.820919	62866	64957	1817-2803	0.800000	77.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	CGGGGGATTGCAAATCCTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.8	58.2	20.3	0.0	0.957130	62866	64957	1773-2759	0.800000	76.3
trnV-GAC-psbJ_p1	trnV-GAC-psbJ	1	AGCCTTGTTCGTCTTTGGTT	GGCGCAGGTCTTCTAAATCA	20	20	45.0	50.0	57.9	58.0	0.0	10.8	0.076958	79093	79825	1425-1585	0.900000	84.8
trnV-GAC-psbJ_p2	trnV-GAC-psbJ	2	TCGACTGATTCAGGGATCGA	GGCGCAGGTCTTCTAAATCA	20	20	50.0	50.0	57.9	58.0	18.5	10.8	0.076958	79093	79825	1681-1855	0.900000	84.4
trnV-GAC-psbJ_p3	trnV-GAC-psbJ	3	TCAGTTCTATTGGGGGCAGA	GGCGCAGGTCTTCTAAATCA	20	20	50.0	50.0	58.0	58.0	0.0	10.8	0.058761	79093	79825	1158-1167	0.800000	73.4
trnV-GAC-psbJ_p4	trnV-GAC-psbJ	4	GTCAGTTCTATTGGGGGCAG	GGCGCAGGTCTTCTAAATCA	20	20	55.0	50.0	58.0	58.0	0.0	10.8	0.068298	79093	79825	1159-1168	0.800000	73.3
trnV-GAC-psbJ_p5	trnV-GAC-psbJ	5	TCAGTTCTATTGGGGGCAGA	AGTGATTCCGTCGTGATTCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.125210	79093	79825	1248-1257	0.800000	72.7
psbJ-ycf3_p1	psbJ-ycf3	1	AAGTGTGGGAAATGGCCAAT	AAAATTTCCATTGCTCCGCG	20	20	45.0	45.0	58.0	58.0	11.0	0.0	0.033646	79949	82340	2556-2560	0.200000	52.0
psbJ-ycf3_p2	psbJ-ycf3	2	AAACGCTAAATTGAAGCCGC	AAAATTTCCATTGCTCCGCG	20	20	45.0	45.0	58.0	58.0	7.3	0.0	0.027896	79949	82340	2902-2903	0.200000	51.7
psbJ-ycf3_p3	psbJ-ycf3	3	TCCTTCCTTAGGGAACTGGG	AAAATTTCCATTGCTCCGCG	20	20	55.0	45.0	58.0	58.0	1.4	0.0	0.038239	79949	82340	2874-2875	0.200000	51.7
psbJ-ycf3_p4	psbJ-ycf3	4	TTGGGCTATGAGCAATGTCC	AAAATTTCCATTGCTCCGCG	20	20	50.0	45.0	57.9	58.0	0.2	0.0	0.062221	79949	82340	2808-2809	0.200000	51.6
psbJ-ycf3_p5	psbJ-ycf3	5	CCGCTTTTTGAGTCCTTCCT	AAAATTTCCATTGCTCCGCG	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.107524	79949	82340	2886-2887	0.200000	51.1
ycf3-rpoB_p1	ycf3-rpoB	1	ATAGCACTTACTCCCGGGAA	CCCTCATTTTGATCCCGGAG	20	20	50.0	55.0	57.8	58.0	26.7	0.7	0.197140	84364	86155	753-2378	1.000000	90.4
ycf3-rpoB_p2	ycf3-rpoB	2	TAGCACTTACTCCCGGGAAT	CCCTCATTTTGATCCCGGAG	20	20	50.0	55.0	57.8	58.0	26.7	0.7	0.197140	84364	86155	752-2377	1.000000	90.4
ycf3-rpoB_p3	ycf3-rpoB	3	ATAGCACTTACTCCCGGGAA	CTCATTTTGATCCCGGAGCA	20	20	50.0	50.0	57.8	58.0	26.7	0.7	0.212698	84364	86155	751-2376	1.000000	90.3
ycf3-rpoB_p4	ycf3-rpoB	4	TAGCACTTACTCCCGGGAAT	CTCATTTTGATCCCGGAGCA	20	20	50.0	50.0	57.8	58.0	26.7	0.7	0.212698	84364	86155	750-2375	1.000000	90.3
ycf3-rpoB_p5	ycf3-rpoB	5	TAGCACTTACTCCCGGGAAT	TCCCTCATTTTGATCCCGGA	20	20	50.0	50.0	57.8	58.4	26.7	0.7	0.579422	84364	86155	753-2378	1.000000	87.4
ndhB-trnI-CAU_p1	ndhB-trnI-CAU	1	CCAGGAATATCAATGAACCCGA	TCCTGTTAAGCATCCATGGC	22	20	45.5	50.0	57.9	57.9	0.0	7.0	2.142495	103225	105621	1505-2534	0.900000	70.9
ndhB-trnI-CAU_p2	ndhB-trnI-CAU	2	CCAGGAATATCAATGAACCCGA	CCTGTTAAGCATCCATGGCT	22	20	45.5	50.0	57.9	57.9	0.0	7.0	2.148328	103225	105621	1504-2533	0.900000	70.8
ndhB-trnI-CAU_p3	ndhB-trnI-CAU	3	TCAATTGCTCAGGATACCCT	TCCTGTTAAGCATCCATGGC	20	20	45.0	50.0	55.9	57.9	0.0	7.0	2.162103	103225	105621	1478-2507	0.900000	70.7
ndhB-trnI-CAU_p4	ndhB-trnI-CAU	4	TCAATTGCTCAGGATACCCT	CCTGTTAAGCATCCATGGCT	20	20	45.0	50.0	55.9	57.9	0.0	7.0	2.167936	103225	105621	1477-2506	0.900000	70.7
ndhB-trnI-CAU_p5	ndhB-trnI-CAU	5	ACCAGGAATATCAATGAACCCG	TCCTGTTAAGCATCCATGGC	22	20	45.5	50.0	58.2	57.9	0.0	7.0	2.236580	103225	105621	1506-2535	0.900000	70.1
trnA-UGC_p1	trnA-UGC	1	TGATTTACTTCACGGGCGAG	GGTGCTGCTAAATGGATGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.061720	117080	117969	1105-1126	1.000000	81.4
trnA-UGC_p2	trnA-UGC	2	TGATTTACTTCACGGGCGAG	AATCATAGATCGAACCGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070575	117080	117969	1061-1082	1.000000	81.4
trnA-UGC_p3	trnA-UGC	3	TGATTTACTTCACGGGCGAG	CATAGATCGAACCGCCGAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.070575	117080	117969	1058-1079	1.000000	81.4
trnA-UGC_p4	trnA-UGC	4	TGATTTACTTCACGGGCGAG	AGGTGCTGCTAAATGGATGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.067553	117080	117969	1106-1127	1.000000	81.3
trnA-UGC_p5	trnA-UGC	5	TGATTTACTTCACGGGCGAG	AGGTGCAATCATATCGCTGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.116368	117080	117969	1028-1044	0.800000	72.8
ndhE-ndhF_p1	ndhE-ndhF	1	CGGATTGATCACGAGTCGAA	CGGGCGCATTTCATCTTATC	20	20	50.0	50.0	57.8	57.4	21.5	10.8	0.790317	140519	140624	290-291	0.200000	41.7
ndhE-ndhF_p2	ndhE-ndhF	2	CGGATTGATCACGAGTCGAA	TCAAATTTATCGGTGGCGGG	20	20	50.0	50.0	57.8	58.6	21.5	0.0	0.804312	140519	140624	306-307	0.200000	41.6
ndhE-ndhF_p3	ndhE-ndhF	3	TACGGATTGATCACGAGTCG	CGGGCGCATTTCATCTTATC	20	20	50.0	50.0	57.2	57.4	11.9	10.8	1.351782	140519	140624	292-293	0.200000	37.2
ndhE-ndhF_p4	ndhE-ndhF	4	TACGGATTGATCACGAGTCG	TCAAATTTATCGGTGGCGGG	20	20	50.0	50.0	57.2	58.6	11.9	0.0	1.365776	140519	140624	308-309	0.200000	37.1
ndhE-ndhF_p5	ndhE-ndhF	5	ACGGATTGATCACGAGTCGA	CGGGCGCATTTCATCTTATC	20	20	50.0	50.0	58.9	57.4	21.5	10.8	1.509133	140519	140624	291-292	0.200000	35.9
ndhF-rpl32_p1	ndhF-rpl32	1	AAAGAAGAAGCCCTACCCCT	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.069094	142845	144744	2733-2787	0.200000	59.4
ndhF-rpl32_p2	ndhF-rpl32	2	AAAGAAGAAGCCCTACCCCT	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070465	142845	144744	2734-2788	0.200000	59.4
ndhF-rpl32_p3	ndhF-rpl32	3	CTTTTTGTTGCGGCTGGAAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.074584	142845	144744	2752-2806	0.200000	59.4
ndhF-rpl32_p4	ndhF-rpl32	4	TTTGTTGCGGCTGGAAAAAG	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.074584	142845	144744	2749-2803	0.200000	59.4
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCGGCTGGAAAAAG	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.075954	142845	144744	2750-2804	0.200000	59.4
ycf1_p1	ycf1	1	ATACTGAAACGGCTGCCATT	CTTCTCCCAAGCCAACAACT	20	20	45.0	50.0	57.9	58.0	20.3	0.0	0.141888	156085	156204	893-1109	1.000000	88.7
ycf1_p2	ycf1	2	CCTCTTTAGGCGTTCTTGCT	CTTCTCCCAAGCCAACAACT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.113722	156085	156204	474-474	0.100000	43.1
ycf1_p3	ycf1	3	CCTCTTTAGGCGTTCTTGCT	ACGAATCAAGGAAGACGTCG	20	20	50.0	50.0	57.9	58.0	0.0	10.5	0.122063	156085	156204	622-622	0.100000	43.0
ycf1_p4	ycf1	4	CCTCTTTAGGCGTTCTTGCT	GAGAGAATGGAACTGCCCAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.136135	156085	156204	174-174	0.100000	42.9
ycf1_p5	ycf1	5	CCTCTTTAGGCGTTCTTGCT	CATGGAGGAATTGGAGACCG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.141563	156085	156204	592-592	0.100000	42.9
rps12-ycf3_p1	rps12-ycf3	1	TAAGAGCGGGGGATTTTGTG	GGTAAAGCGGAAGAAGCCTT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.300277	163043	163974	397-1930	1.000000	88.3
rps12-ycf3_p2	rps12-ycf3	2	TAAGAGCGGGGGATTTTGTG	ATAGCACTTACTCCCGGGAA	20	20	50.0	50.0	57.9	57.8	0.0	26.7	0.292795	163043	163974	438-1971	1.000000	88.2
rps12-ycf3_p3	rps12-ycf3	3	TAAGAGCGGGGGATTTTGTG	TAGCACTTACTCCCGGGAAT	20	20	50.0	50.0	57.9	57.8	0.0	26.7	0.292795	163043	163974	437-1970	1.000000	88.2
rps12-ycf3_p4	rps12-ycf3	4	GAGGGCATCTCTTAAGAGCG	ATAGCACTTACTCCCGGGAA	20	20	55.0	50.0	57.9	57.8	25.0	26.7	0.317448	163043	163974	450-1983	1.000000	88.0
rps12-ycf3_p5	rps12-ycf3	5	GAGGGCATCTCTTAAGAGCG	TAGCACTTACTCCCGGGAAT	20	20	55.0	50.0	57.9	57.8	25.0	26.7	0.317448	163043	163974	449-1982	1.000000	88.0
trnA-UGC_p1	trnA-UGC	1	GGTGCTGCTAAATGGATGGA	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.061720	171716	172605	1105-1126	1.000000	81.4
trnA-UGC_p2	trnA-UGC	2	AATCATAGATCGAACCGCCG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070575	171716	172605	1061-1082	1.000000	81.4
trnA-UGC_p3	trnA-UGC	3	CATAGATCGAACCGCCGAAT	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.070575	171716	172605	1058-1079	1.000000	81.4
trnA-UGC_p4	trnA-UGC	4	AGGTGCTGCTAAATGGATGG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.067553	171716	172605	1106-1127	1.000000	81.3
trnA-UGC_p5	trnA-UGC	5	AGGTGCAATCATATCGCTGG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.116368	171716	172605	1028-1044	0.800000	72.8